Hi @ebolyen, I cannot reproduce the same problem with the moving pictures tutorial.
I started running both my data and the moving pictures in two different terminals. The output of the tutorial looks fine:
Last login: Wed Jan 3 14:13:16 on ttys000
dhcp-morris-2820:~ ErikaGanda$ cd /Users/ErikaGanda/Desktop/qiime2-moving-pictures-tutorial
dhcp-morris-2820:qiime2-moving-pictures-tutorial ErikaGanda$ ls
demux.qza emp-single-end-sequences
demux.qzv sample-metadata.tsv
dhcp-morris-2820:qiime2-moving-pictures-tutorial ErikaGanda$ source activate qiime2-2017.12
(qiime2-2017.12) dhcp-morris-2820:qiime2-moving-pictures-tutorial ErikaGanda$ qiime dada2 denoise-single \
--i-demultiplexed-seqs demux.qza
--p-trim-left 0
--p-trunc-len 120
--o-representative-sequences rep-seqs-dada2.qza
--o-table table-dada2.qza
Saved FeatureTable[Frequency] to: table-dada2.qza
Saved FeatureData[Sequence] to: rep-seqs-dada2.qza
(qiime2-2017.12) dhcp-morris-2820:qiime2-moving-pictures-tutorial ErikaGanda$
My data is still running. If I get another error I will post here. I went back and my demultiplexing files look ok:
rumen-demux.qzv (283.5 KB)
A while ago I had DADA2 give me errors and @jairideout helped me in this post: DADA2 error (return code -5) - #10 by jairideout
Maybe something similar is going on this time around?
Thanks again!!