DADA2 Error (return code 1)

Hi @cjweiss,

Thanks for posting!

Apparently you have an unequal number of sequences in your forward and reverse sequence files. See this thread for a similar error and some suggestions for diagnosing the error (including, near the bottom of the thread, a way to find the problem sequences):

Thanks for trying these, but alas that's not the issue — you either imported the wrong files, or these files just have unequal seq numbers for some other reason (e.g., something was trimmed or corrupted). So these files are fine and will validate — they just don't match in the way that they need to (i.e., the forward read is missing its reverse pair :cry: )

I hope that helps! Let us know if you are able to identify the problem!