Hi,
I know that this question is similar to some other postings, however, I have tried using the suggestions in those posting to resolve my error and have been unable to do so. I received the following error while trying to run DADA2:
An error was encountered while running DADA2 in R (return code 1), please inspect stdout and stderr to learn more.
The log file states the following:
Running external command line application(s). This may print messages to stdout and/or stderr.
The command(s) being run are below. These commands cannot be manually re-run as they will depend on temporary files that no longer exist.
Command: run_dada_paired.R /tmp/tmpl4w_6vh6/forward /tmp/tmpl4w_6vh6/reverse /tmp/tmpl4w_6vh6/output.tsv.biom /tmp/tmpl4w_6vh6/filt_f /tmp/tmpl
4w_6vh6/filt_r 221 233 19 20 2.0 2 consensus 1.0 20 1000000
R version 3.4.1 (2017-06-30)
Loading required package: Rcpp
DADA2 R package version: 1.6.0
- Filtering Error in filterAndTrim(unfiltsF, filtsF, unfiltsR, filtsR, truncLen = c(truncLenF, :
These are the errors (up to 5) encountered in individual cores…
Error in (function (fn, fout, maxN = c(0, 0), truncQ = c(2, 2), truncLen = c(0, :
Mismatched forward and reverse sequence files: 17737, 17732.
Error in (function (fn, fout, maxN = c(0, 0), truncQ = c(2, 2), truncLen = c(0, :
Mismatched forward and reverse sequence files: 15024, 15022.
Error in (function (fn, fout, maxN = c(0, 0), truncQ = c(2, 2), truncLen = c(0, :
Mismatched forward and reverse sequence files: 17737, 17732.
Error in (function (fn, fout, maxN = c(0, 0), truncQ = c(2, 2), truncLen = c(0, :
Mismatched forward and reverse sequence files: 15024, 15022.
Error in (function (fn, fout, maxN = c(0, 0), truncQ = c(2, 2), truncLen = c(0, :
Mismatched forward and reverse sequence files: 17737, 17732.
Execution halted
Traceback (most recent call last):
File “/s/angus/index/common/tools/miniconda3/envs/qiime2-2018.2/lib/python3.5/site-packages/q2_dada2/_denoise.py”, line 179, in denoise_paire
d
run_commands([cmd])
File “/s/angus/index/common/tools/miniconda3/envs/qiime2-2018.2/lib/python3.5/site-packages/q2_dada2/_denoise.py”, line 35, in run_commands
subprocess.run(cmd, check=True)
File “/s/angus/index/common/tools/miniconda3/envs/qiime2-2018.2/lib/python3.5/subprocess.py”, line 398, in run
output=stdout, stderr=stderr)
subprocess.CalledProcessError: Command ‘[‘run_dada_paired.R’, ‘/tmp/tmpl4w_6vh6/forward’, ‘/tmp/tmpl4w_6vh6/reverse’, ‘/tmp/tmpl4w_6vh6/output.
tsv.biom’, ‘/tmp/tmpl4w_6vh6/filt_f’, ‘/tmp/tmpl4w_6vh6/filt_r’, ‘221’, ‘233’, ‘19’, ‘20’, ‘2.0’, ‘2’, ‘consensus’, ‘1.0’, ‘20’, ‘1000000’]’ re
turned non-zero exit status 1
During handling of the above exception, another exception occurred:
Traceback (most recent call last):
File “/s/angus/index/common/tools/miniconda3/envs/qiime2-2018.2/lib/python3.5/site-packages/q2cli/commands.py”, line 246, in call
results = action(**arguments)
File “”, line 2, in denoise_paired
File “/s/angus/index/common/tools/miniconda3/envs/qiime2-2018.2/lib/python3.5/site-packages/qiime2/sdk/action.py”, line 228, in bound_callabl
e
output_types, provenance)
File “/s/angus/index/common/tools/miniconda3/envs/qiime2-2018.2/lib/python3.5/site-packages/qiime2/sdk/action.py”, line 363, in callable_exe
cutor
output_views = self._callable(**view_args)
File “/s/angus/index/common/tools/miniconda3/envs/qiime2-2018.2/lib/python3.5/site-packages/q2_dada2/_denoise.py”, line 194, in denoise_paire
d
" and stderr to learn more." % e.returncode)
Exception: An error was encountered while running DADA2 in R (return code 1), please inspect stdout and stderr to learn more.
I have tried re-importing. I have double checked and even re-created the manifest file. I have also validated the demux file.
Any help would be greatly appreciated!
Thanks,
Carla