DADA2 Error rates could not be estimated -- but I don't know why

[colinbrislawn (Colin J Brislawn)]

Hello Norma,

Welcome to the forums!

Here is the import lines of the error:

@Norma

16295750 total bases in 65183 reads from 1 samples will be used for learning the error rates.
Error rates could not be estimated (this is usually because of very few reads).
Error in getErrors(err, enforce = TRUE) : Error matrix is NULL.

This has happened before when dealing with a small number of samples and NovaSeq data.

Do you have more samples?

Is this Illumina NovaSeq?