DADA2 Error: No reads passed filter

Okay, this is very unusual, since your reads are very good quality and plenty long!

I think I have found the issue:

It looks like perhaps this is a permissions issue, and you cannot write files to the default temp directory that dada2 is attempting to access. Chances are if you ran this on your own computer all would work, but since you are using a remote environment (cyverse) this issue is occurring.

One way to get around this may be to define a different temporary directory to which you do have write permission. See here:

You should probably get in contact with the system admin to discuss permissions and what temp directory to use.

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