I also have problem with another dada2 run for 730 paired-end forward reads as follows
qiime dada2 denoise-single --i-demultiplexed-seqs pe_f-demux.qza --output-dir dada2_f/ --p-trunc-len 280 --p-trim-left 20 --p-n-threads 16
Plugin error from dada2:
An error was encountered while running DADA2 in R (return code 1), please inspect stdout and stderr to learn more.
Debug info has been saved to /tmp/qiime2-q2cli-err-n7gkm2ku.log
(qiime2-2019.4) ashutosh@fisher:~/data/$ less /tmp/qiime2-q2cli-err-n7gkm2ku.log
Running external command line application(s). This may print messages to stdout and/or stderr.
The command(s) being run are below. These commands cannot be manually re-run as they will depend on temporary files that no longer exist.
Command: run_dada_single.R /tmp/qiime2-archive-ao0pdm85/d57de86e-9a19-4c2d-8b94-1c9855e7180e/data /tmp/tmpd9noj5qg/output.tsv.biom /tmp/tmpd9noj5qg/track.tsv /tmp/tmpd9noj5qg 280 20 2.0 2 Inf consensus 1.0 16 1000000 NULL 16
R version 3.5.1 (2018-07-02)
Loading required package: Rcpp
DADA2: 1.10.0 / Rcpp: 1.0.1 / RcppParallel: 4.4.2
1) Filtering The filter removed all reads: /tmp/tmpd9noj5qg/SRX1617839_75_L001_R1_001.fastq.gz not written.
The filter removed all reads: /tmp/tmpd9noj5qg/SRX1617905_91_L001_R1_001.fastq.gz not written.
The filter removed all reads: /tmp/tmpd9noj5qg/SRX1617843_78_L001_R1_001.fastq.gz not written.
The filter removed all reads: /tmp/tmpd9noj5qg/SRX1617954_94_L001_R1_001.fastq.gz not written.
The filter removed all reads: /tmp/tmpd9noj5qg/SRX1617841_76_L001_R1_001.fastq.gz not written.
and the end of the log file is as follows
The filter removed all reads: /tmp/tmpd9noj5qg/SRX3660940_352_L001_R1_001.fastq.gz not written.
The filter removed all reads: /tmp/tmpd9noj5qg/SRX3660956_368_L001_R1_001.fastq.gz not written.
The filter removed all reads: /tmp/tmpd9noj5qg/SRX5453580_656_L001_R1_001.fastq.gz not written.
Error in filterAndTrim(unfilts, filts, truncLen = truncLen, trimLeft = trimLeft, :
These are the errors (up to 5) encountered in individual cores...
Error in writeFastq(fq, fout, "a", compress = compress) :
failed to write record 18184
Error in writeFastq(fq, fout, "a", compress = compress) :
failed to write record 18184
Error in writeFastq(fq, fout, "a", compress = compress) :
failed to write record 18184
Error in writeFastq(fq, fout, "a", compress = compress) :
failed to write record 18184
Error in writeFastq(fq, fout, "a", compress = compress) :
failed to write record 18184
Execution halted
Traceback (most recent call last):
File "/home/ashutosh/anaconda2/envs/qiime2-2019.4/lib/python3.6/site-packages/q2_dada2/_denoise.py", line 152, in _denoise_single
run_commands([cmd])
File "/home/ashutosh/anaconda2/envs/qiime2-2019.4/lib/python3.6/site-packages/q2_dada2/_denoise.py", line 36, in run_commands
subprocess.run(cmd, check=True)
File "/home/ashutosh/anaconda2/envs/qiime2-2019.4/lib/python3.6/subprocess.py", line 418, in run
output=stdout, stderr=stderr)
subprocess.CalledProcessError: Command '['run_dada_single.R', '/tmp/qiime2-archive-ao0pdm85/d57de86e-9a19-4c2d-8b94-1c9855e7180e/data', '/tmp/tmpd9noj5qg/output.tsv.biom', '/tmp/tmpd9noj5qg/track.tsv', '/tmp/tmpd9noj5qg', '280', '20', '2.0', '2', 'Inf', 'consensus', '1.0', '16', '1000000', 'NULL', '16']' returned non-zero exit status 1.
During handling of the above exception, another exception occurred:
Can you tell me what is going on
Thanks In advance
Ashutosh