@colinbrislawn Yes this is a large NovaSeq run and after importing the data in qiime, it is giving me 110Gb file. I have 32 samples (~3-4gb each). I am struggling on this DADA2 step since last moth still no progress. Last time I asked (Can removal of RNA-seq human reads expedite the analysis? (Especially DADA2)) about individual sample analysis.
Can I analyze samples in 2-3 batches and later merge the feature table?
" Processing your 100GB files in chunks could solve your storage issue, and will also give you better results!"
What is the command to do this ? is it simple splitting of file?
Kindly help me to troubleshoot the analysis for this data. In upcoming months I have to analyze more batches of samples.