I have just recently started working with qiime2 (version 2020.8), and I am trying to work through the initial steps using my old paired-end fastaq files. The forward and reverse files were already demultiplexed from the person who sequenced them. I was able to import them without a problem, but I started to run into issues when running the dada2 denoise-paired code. Below is the code that I used, from the import to the error I get:
qiime tools import \
Imported qiime-import-practice as PairedEndFastqManifestPhred33V2 to testing_file.qza
qiime demux summarize \
Saved Visualization to: demux-summary-test.qzv
qiime dada2 denoise-paired \
Plugin error from dada2:
No features remain after denoising. Try adjusting your truncation and trim parameter settings.
Debug info has been saved to /var/folders/mq/dv8ks0ns3pgc0jy9ltrnf7p80000gn/T/qiime2-q2cli-err-31b72ezf.log
I tried adjusting the parameters like it says, even having both at zero, and I get the same error. I am worried that it may be either the formatting of my fastaq files, or that the quality of them is just too low to do this. Below is one sequence from a forward (top) and reverse (bottom) read of a sample. Additionally, I have added the demux summary image in case that is of help in figuring out this problem:
[email protected][email protected]?FCFGGGFFFDFFDFGGEEEGCD<BBFGGGGFEFCFFFA9CBDFFCABFF9BFF,@FDG<[email protected]EFGGGGEG:BE:::,?FFGEFE?FFCF7?<<<BCCEC;EE??7BCFDDEG>C+8<4?>[email protected];CGGG?7<:<)9BCC;(;5+:8CB?FF:>:D8:>99:>9:(:7F?FF7+5:>>?+<+.2;([email protected]<A,7)440,.
(I hope these upload right!)
Any help would be greatly appreciated! If any other information you would think could be helpful I can try and provide you.
Thank you in advance for your time!