Hello Matt, So I am at the point where I am trimming my sequences based on the demux.qzv plot but I received an error message in red font and it states that a debug message has been saved in a temp folder. I am pasting in my command and the error message below it. Please advise... Lisa qiime dada…

Hi @Lisa_Crummett , thanks for posting the error log. We have seen this "exit status -9" error crop up a few times lately --- it is an error from DADA2, not from QIIME 2. It is a bit of a catch-all error, but generally what we have seen is that this most often happens when the computer that you are r…

Hi @Lisa_Crummett , Could you follow up with the log file (/tmp/qiime2-q2cli-err-5qheqfod.log)? When DADA2 returns -9 it usually means something else killed it (often because it ran out of memory). Thanks!

Hello Evan, I could not find the log file. It didn't show up in the directory that I was working in and I didn't see a "temporary" folder listed in the folder icon to the left. How do I access this file? I am off campus today so I may not be able to send it until Monday unfortunately.

Hey @Lisa_Crummett , [image] Lisa_Crummett: I could not find the log file. It didn’t show up in the directory that I was working in and I didn’t see a “temporary” folder listed in the folder icon to the left. It is at the root of your filesystem, I'm guessing you are on a Ubuntu linux distro,…

Hi Evan, So, I tried tha the command that you suggested and got this error: cat: /tmp/qiime2-q2cli-err-5qheqfod.log: No such file or directory I found the "root" folder but could not open it. I found a "tmp" folder and the only thing that looked like it could be a log is called, "/tmp/config-err…

So I tried running the same command again and now I get errors saying that each line in the command is "not found". I also running Data2 denoise in Qiime2 Studio and after I click"Go", nothing happens and it says that there are "no jobs" running. What is going on?? Could I talk to one of you guys ov…

Okay, now I have the error log file for the most recent time that I tried running the Dada2 command. Here it is... Running external command line application(s). This may print messages to stdout and/or stderr. The command(s) being run are below. These commands cannot be manually re-run as they wil…

Hi Matt, Thank you for your feedback. I tried increasing the amount of RAM allocated to VirtualBox (I increased it to 4645 MB) and then ran the Dada2 denoising command and it has been running for over 24 hours now with no response. I'm not sure what to do at this point. Do you know how many MB of R…

Hi @Lisa_Crummett : [image] Lisa_Crummett: running for over 24 hours now with no response Real-world data-sets can take a bit of time on this step. Fortunately, once it is done a lot of the down-stream steps are pretty quick, relatively speaking! [image] Lisa_Crummett: I’m not sure what …

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