Dada2 denoise error message

[moryfranklin22 (Mory)]

Hello,

After I ran the following denoise method I received the dada2 error message below and was unable to find any related forum posts. The method ran for about two hours and then aborted. I used qiime2017.2.

$ qiime dada2 denoise-paired \
--i-demultiplexed-seqs demux-paired-end.qza \
 --o-table table \
 --o-representative-sequences rep-seqs \
--p-trim-left-f 0 \
 --p-trim-left-r 0 \
 --p-trunc-len-f 225 \
 --p-trunc-len-r 225 \
 --p-n-threads 0 \
--p-n-reads-learn 10000

Plugin error from dada2:

  Command '['run_dada_paired.R', '/tmp/tmpk0jsxbc_/forward',
  '/tmp/tmpk0jsxbc_/reverse', '/tmp/tmpk0jsxbc_/output.tsv.biom',
  '/tmp/tmpk0jsxbc_/filt_f', '/tmp/tmpk0jsxbc_/filt_r', '225', '225',
  '0', '0', '2.0', '2', '0', '1000000']' returned non-zero exit status 1

Re-run with --verbose to see debug info.

[thermokarst]

Hi @moryfranklin22, can you please rerun that command with the --verbose flag included, and then post the output here?

 $ qiime dada2 denoise-paired \
  --i-demultiplexed-seqs demux-paired-end.qza \
  --o-table table \
  --o-representative-sequences rep-seqs \
  --p-trim-left-f 0 \
  --p-trim-left-r 0 \
  --p-trunc-len-f 225 \
  --p-trunc-len-r 225 \
  --p-n-threads 0 \
  --p-n-reads-learn 10000 \
  --verbose