Hello everyone, I am working through the QIIME/microbiome sequence process for the first time and I've gotten hung up on a few questions on my DADA2 results. I am working with 2x251 paired end sequences of the V4 region, with 515F and 806R primers. The sequencing followed the EMP protocol, so the …

Hi Mike, Thanks for reaching out, happy to provide some insight on these dada2 results! I first wanted to discuss the difference between p-trunc-len and p-trim (as this may help clarify your differing results): p-trunc-len: Position at which read sequences (forward or reverse) should be truncated…

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DADA2: Decreasing feature number as more sequences are maintained