after cutadapt step we have removed adapters and primers. why do we use trim left in dada2 step? sorry about it if it sounded irrelevent but im new to this

Hi @the_dummy , Welcome to the forum! I moved your question over to a more general post in user support. Best, Justine

Hi @the_dummy , Welcome to the forum and good inquisitive question. The trim left option in dada2 can be used to either trim adapters (as you have done in vsearch) or remove low quality portions of your reads which sometimes occur during the first 10-20 bp on the 5' end. This is not uncommon from I…

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Cutadapt trimming in ITS tutorial

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