Cutadapt primers orientation and further steps

[sdpapet]

@timanix

qiime cutadapt trim-paired \
    --i-demultiplexed-sequences demux.qza \
    --o-trimmed-sequences trimmed.qza \
    --p-cores 6 \
    --p-front-f CAAGRGTTHGATYMTGGCTCAG \
    --p-front-r TGCTGCCTCCCGTAGGAGT \
    --p-match-adapter-wildcards \
    --p-discard-untrimmed \
    --p-match-read-wildcards

Thank you very much for your script. I read this script and I have a few questions.

1> Is this script will remove the primers (provided) and any preceding sequenses (including barcodes in that case)? If so, --p-front-f and --p-front-r parameters will do this work. Am I correct?

2> I also notice that there are other parameters such as --p-adapter-f and --p-adapter-r.
What is the difference between --p-front-f and --p-adapter-f ? I read the manual. It seems --p-adapter-f and --p-adapter-r are for the reads with adapters/barcodes on both forward and reverse reads. Should I also provide my reverse compliment primer sequences by using --p-adapter-f and --p-adapter-r, just in case (as I don't know how the sequencing arrange the adaptors)?

If I do need to add these parameters, --p-adapter-f should be reverse complement sequence of my REVERSE primer and --p-adapter-r should be reverse complement sequence of my FORWARD primer?

Or --p-adapter-f should be reverse complement sequence of my FORWARD primer and --p-adapter-r should be reverse complement sequence of my REVERSE primer.

3>After I run remove barcodes and sequences using cutadapt. I need to run DADA2. Usually, it will still have low quality score bases at the beginning or end of the sequences. I need to cut off these. Normally, what Q-score do you choose? 30, 35 or 40?

Thank you