I'm am using qiime2-2022.2 and have denoised my samples using DADA2. I did not truncate my seqs and wanted to use the latest SILVA database (13.8.1) with full length seqs (not NR99). The format of the database has completely changes since 13.2 so I am at a loss on which files to choose and how to im…

[image] sraza: wanted to use the latest SILVA database (13.8.1) with full length seqs (not NR99). The format of the database has completely changes since 13.2 so I am at a loss on which files to choose and how to import the database correctly. Hi @sraza , You can download pre-made SILVA class…

silva-138-99-tax.qza (6.5 MB) Hi @SoilRotifer , Thanks so much for this information. I downloaded the pre-made QZA file (silva-138-99-tax.qza & silva-138-99-seqs.qza) and I also made my own reference database as per the instructions (RESCRPt plugin) after importing the taxa file I unzipped it to c…

[image] sraza: Now sure what I'm doing wrong ? Hi @sraza , you are not doing anything wrong. To make the pre-made files, we basically followed the procedure outlined in the RESCRIPt tutorial. If you made the database yourself, using either the same or different parameters, it is quite possibl…

QIIME 2 Forum

Correct way to import SILVA 13.8.1 database into qiime2

Technical Support

show post in topic

Home
Categories
Guidelines
Terms of Service
Privacy Policy

Powered by Discourse, best viewed with JavaScript enabled