Hello,
I reached the visualization step of the post-dada2 phase of my seqs.
Since I already have multiplexed files I used a manifest.txt file to create a single qza artifact with all of the 8 samples. I ran the dada2 method, and now would like to use this command:
qiime feature-table summarize \
--i-table table.qza
--o-visualization table.qzv
--m-sample-metadata-file manifest.txt
However, it seems that the --m sample-metadata-file do not accept the manifest file as a metadata, and I get this error:
There was an issue with loading the file manifest.txt as metadata:
Invalid characters (e.g. '/', '\x00', '\', '*', '<', '>', '?', '|', '$') or empty ID detected in metadata index: 'BS3,/home/eddie/Barak/banana/eddie_single_end/BS3.fastq,forward'. There may be more errors present in this metadata. Sample/feature metadata files can be validated using Keemei: http://keemei.qiime.org
I do understand the literal meaning of the error, but how can I create a metadata file in Excel that will correspond to the manifest file, so I won't lose any information and that will match my table.qza file?
Attached is my manifest.txt file.
Thank you!
Barakmanifest.txt (624 Bytes)