Hi @azithro13,
The metadata from the QIIME 2 Moving Pictures tutorial is specifically for the associated data in that tutorial. Your metadata file should contain information on your specific dataset. I would recommend reaching out to your colleague who provided you with all of these files and request a metadata file for your data specifically.
Regarding the longitudinal volatility plot you shared with me via direct message - this plot is difficult to interpret because your dataset is not a longitudinal study that took place over a certain number of days/months, etc. Based on what you've shared with me, all of your samples were taken on the same day - meaning you don't have any metrics to measure changes over time.
Please refer to our installation guide for utilizing the latest version of QIIME 2 via Docker:
https://docs.qiime2.org/2023.2/install/virtual/docker/
Cheers 