Confirmation on code that removes Homopolymers

[Nicholas_Bokulich]

Hi @tamardigrade ,

See here:

Processing, filtering, and evaluating the SILVA database (and other reference sequence data) with RESCRIPt

Please consider this tutorial a living document, which may change based upon community feedback and ongoing plugin development. RESCRIPt RESCRIPt (REference Sequence annotation and CuRatIon Pipeline) is a python package and QIIME 2 plugin for formatting, managing, and manipulating sequence reference databases. This package was designed for compiling, manipulating, and evaluating sequence reference databases from SILVA, NCBI, Greengenes, GTDB, and other sources, and for constru…

The cull-seqs action is part of the RESCRIPt plugin, which must be installed separately. Note that this will only remove homopolymer from FeatureData[Sequence] or RNASequence artifacts, i.e., after reads have been denoised or clustered.

Good luck!