Compressed file ended before the end-of-stream marker was reached

User Support
1

Hi,

I'm new to QIIME2 and was trying out the "Moving-Pictures" tutorial. I have updated to the most recent version of QIIME2. When trying to demultiplex the single end sequences:

qiime demux emp-single
--i-seqs emp-single-end-sequences.qza
--m-barcodes-file sample-metadata.tsv
--m-barcodes-category BarcodeSequence
--o-per-sample-sequences demux.qza

I get the same error:

Plugin error from demux:
Compressed file ended before the end-of-stream marker was reached

Any suggestions?

3

Hi @sbudree!

Could you run qiime tools peek on your emp-single-end-sequences.qza file? I don't really expect that to fail, but let me know if you see the same error.

I am guessing your fastq.gz files that were imported are corrupt somehow. You could try downloading them again, it was likely just a network error/blip that messed up the file transfer.

Let me know if that fixes it!