I’m new to QIIME2 and was trying out the “Moving-Pictures” tutorial. I have updated to the most recent version of QIIME2. When trying to demultiplex the single end sequences:
Could you run qiime tools peek on your emp-single-end-sequences.qza file? I don’t really expect that to fail, but let me know if you see the same error.
I am guessing your fastq.gz files that were imported are corrupt somehow. You could try downloading them again, it was likely just a network error/blip that messed up the file transfer.