Hi @SY_Yang,
512 Gig RAM should certainly be enough, though in your bash script I'm seeing you only requesting 128 Gig, which should still be fine... I'm guessing there's something happening with you actually accessing that available RAM across the server. While someone with more experience with these helps you out, could you instead use this pre-trained classifier of the V3-V4 region with Silva 132 (newer than 128)?
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