Hi @MMC_northS,
thanks for replying. I have sequences of the 16S done with the Ion 16S Metagenomics kit, which are formed by two sets of primers: 1) V2-4-8, 2) V3-6,7-9. My problem is that I'd love train my data with classify-sklearn, but I need the primer sequences that are not published. This is my problem.
As you say, there is an specific data to analyze the data in the ion reporter, but it has a lot of limitations, as you can't introduce the experimental groups or any other parameter. With QIIME1, you can use a limited number of scripts with the files from ion reporter, I couldn't try with QIIME2 yet. Next step. I love it, because they use a curated database and you obtain the most of the species level, but I need to work on that...
Many thanks!
Isabel