Hi!
I have a query regarding the metadata file-it's more of a conceptual question, and I would like to clear my fundamental understanding before going into deeper analyses.
The metadata file has categorical and numeric columns, and for some analyses such as diversity, gneiss etc., the input only allows categorical or numeric columns (depends on the plugin). If suppose I wish to analyse an attribute (say Nitrogen dose, a column in my metadata file) which I have denoted as 'categorical' whereas the particular plugin only requires 'numeric':
a) Can I re-run the analysis by removing alphabet characters in all the rows under that attribute and renaming the attribute category as 'numeric'? I know the plugin will run, but would my results be inaccurate? This is because I wish to see how trends will appear with respect to the Nitrogen dose.
b) Will this deem previous analyses (e.g. rarefaction, diversity, taxonomy etc.) which I have done with 'Nitrogen dose' denoted as 'categorical' to be inaccurate?
PS: I denote the rows under 'Nitrogen dose' as a numerical value by only signifying the magnitude of the dose in the metadata file. If I wish to make it appear as a categorical value, I simply attach "ppm" at the end of the magnitude value.
The macro-question would be:
Can I make changes in the metadata file during my analyses, where certain scripts/plugins take input the metadata in a format which is different from that taken input by previous scripts/plugins?
Thanks a lot for your help.
Best regards,
Anirban