Hello all! I have
SampleData[PairedEndSequencesWithQuality]
that I'm looking to demultiplex but I am receiving the following error.
qiime demux emp-paired --m-barcodes-file sample-metadata.tsv --m-barcodes-column BarcodeSequence --i-seqs seqs.qza --o-per-sample-sequences demux-full.qza --o-error-correction-details demux-error.qza
Is it possible to change the format of my seqs?
Hi @jzbuser,
Can you please post the full error? What you have shared is only the command that would have caused the error not the error message itself. A screenshot works!
--Hannah
Hello, thank you for your help. Sorry I didn't post the error!
(1/1) Invalid value for '--i-seqs': Expected an artifact of at least type
EMPPairedEndSequences. An artifact of type
SampleData[PairedEndSequencesWithQuality] was provided.
Hi!
Based on the error here it is most likely that your sequences are already demultiplexed, so you can go ahead and skip this step!
If this is not the case, I would recommend searching the forum for this issue and looking at how other users have resolved this issue in the past. There is a ton of useful information to be found there!
--Hannah