Cannot upload files to qiime2

Hi everyone, I am new to qiime and I am struggling so much on how to upload my sequences to qiime2.

So I have generated a manifest file as below, and I tried to import my sequence with the code:

qiime tools import \

--type 'SampleData[PairedEndSequencesWithQuality]'
--input-path ~/siyu/scratch/miseq_lrx/files/manifest.csv
--output-path demux.qza
--input-format PairedEndFastqManifestPhred33V2

However, everytime I run the code it gives me the error
(1/1) Invalid value for '--input-path': Path 'manifest.csv' does not exist.

I have made sure the manifest.csv file is in the correct path, and have tried both absolute and relative path but nothing worked. What could go wrong here?

manifest.csv (22.1 KB)

Hello @Siyu_Song,

Are you sure that the file manifest.csv is in the same directory as where you ran that command?

Sorry, didn't notice that you had provided a full path to the command. Have you tried using an absolute path to that file?

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