Hi everyone, I am new to qiime and I am struggling so much on how to upload my sequences to qiime2.
So I have generated a manifest file as below, and I tried to import my sequence with the code:
qiime tools import \
--type 'SampleData[PairedEndSequencesWithQuality]'
--input-path ~/siyu/scratch/miseq_lrx/files/manifest.csv
--output-path demux.qza
--input-format PairedEndFastqManifestPhred33V2
However, everytime I run the code it gives me the error
(1/1) Invalid value for '--input-path': Path 'manifest.csv' does not exist.
I have made sure the manifest.csv file is in the correct path, and have tried both absolute and relative path but nothing worked. What could go wrong here?
manifest.csv (22.1 KB)