Cannot import FASTA file for Sanger sequences

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1

I have successfully imported these types of data in the past using this command, but when I try to do that now, I get the error

There was a problem importing Hines_SeqData_Final.fasta:
Hines_SeqData_Final.fasta is not a(n) QIIME1DemuxFormat file

I did clean up/simplify my sequences names before trying to import this time. Would that cause this error, or is this an issue stemming from the new q2-2019.1 release?

Here is a sample of some of my sequences:

HCl.49
TGGTTNTGGCATCGATGAAGAACGCAGCGAAATGCGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACATTGCGCCCTCTGGTATTCCGGGGGGCATGCCTGTTCGAGCGTCATTACAACCCTCAAGCTNTGCTTGGTATTAGGTTTCACCCGTAAGGGCGTACCGTAAAACTAGTGGCGGTGCCNTTCGGCTTCNAGCGTAGTAATTCTTCTCGCTTTGAACNCCGGTTGAGTGCTTGCCAACAACCCCAATTTTTATCAAAGGTTGACCTCGGATCAGGTAGGGATACCCGCTGA
HCl.50
TGGTTCTGGCATCGATGAANAACGCANCGAAATGCGATAAGTAATGTGAATTGCAAAATTCAGTGAATCATCGAATCTTTGAACGCACATTGCGCCCTGTGGTATTCCGCAGGGCATGCCTGTTCGAGCGTCATTTCAACCCATCAAGCTTACGCTTGGTATTGGGGCCTGCGGTTTCGCAGCCTCTAAACTCAGTGGCGGTGCTATTGAGCTCTGAGCGTAGTAATTTTTCTCGCTATAGGGTCTCGGTAGTTACTTGCCAGTAACCCCCAATTTTTATCAGGTTGACCTCGGATCAGGTAGGGATACCCGCTGA

Any help is much appreciated.

2

Hi @jhines1! The QIIME1DemuxFormat is defined here:

http://qiime.org/documentation/file_formats.html#post-split-libraries-fasta-file-overview

Looks like your label lines need to be updated to match that spec. Are the .49 and .50 sequences IDs? If so, you can just replace the periods . with spaces.

3

EDIT: Replacing the ‘.’ with ‘_’ worked. Thanks!!

4

No need to apologize - I confused myself re-reading that spec. You should actually use an underscore _, not a space (I think).

5

It worked. Thanks!! Glad it was that simple! :grin:

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