building a (newer!) COI database from NCBI

Hi, Thanks for giving us in details. However, I wonder that your post is written in 2020. But, I think in these year the dataset would be included more than 2020. In these case, how could I download it? In this case, do I have to use the option 2? Further refining the reference sequences: creating primer-coordinate defined databases? and one more questions, how could I integrate the both NCBI and BOLD databases at one file?

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Hello @Soyeon_Kim95,

Welcome to the forums! :qiime2:

(I've split your question into a new post.)

The RESCRIPt plugin discussed earlier still works great for building custom databases.

Yes, NBCI and BOLD are both supported by rescript!

As you work through those tutorials, let us know if you have any questions.