Hi everyone, I need some help to interpret the beta diversity results better. I am especially confused by Bray-Curtis results when compared with weighted, unweighted and taxonomic analysis. In Bray-Curtis, I observe a clear clustering pattern by library preparation plate: samples from plate 1 clus…

Hi @AnnaC , I think your interpretation is on the right track: [image] AnnaC: I feel that same bacterial species are classified to different ASVs, maybe driving this divergence in Bray-Curtis, which is lost when using phylogeny-aware metrics. With Bray-Curtis, you weight the difference betwe…

Thanks, @jwdebelius !!! I think it might be this. The ASVs, which are giving the difference among clusters, are identical in the exception of a single base in the beginning and/or in the end. We are trimming the primers by length since we do not have the sequence (commercial primers), so I think the…

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Bray curtis PCoA vs UniFrac interpretation

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