When running beta diversity on my ITS data
qiime diversity beta-group-significance
Plugin error from diversity:
All values in the grouping vector are unique. This method cannot operate on a grouping vector with only unique values (e.g., there are no ‘within’ distances because each group of objects contains only a single object).
Debug info has been saved to /tmp/qiime2-q2cli-err-wgawlcud.log
However, I have run this before on my 16s data and has run fine (the same principle of the metadata).
There are two samples in my data which are the only ones with individual “description” names (if you check these for duplicates), while all the others appear at least twice. I am wondering if this is the cause of my error.
I would like to, in theory, run the command ignoring this problem and not having to remove the samples entirely and re-run stats as I have already started analyzing the other statistics. I can always adjust my downstream to ignore the pairwise with low sample sizes.
Let me know if this makes sense or if you need more information. I am attaching my metadata file as well.
meta_ITS.txt (6.3 KB)