Best approach for analyzing Sanger sequences from ITS2 region

[thermokarst]

Hey there @jhines1!

@jhines1

Would this give vsearch, or Q2 in general, reasons to throw errors?

I don't think that is the problem here (but I could be wrong...)

@jhines1

observation_id = fields[9].split()[0]

IndexError: list index out of range

One of these list lookups here [9], [0] is failing because the field doesn't exist.

The FASTA file snippet you provided above might not satisfy the format spec --- what happens when you run the following?

qiime tools validate Hines_SeqData.qza

Thanks!