barcode identifiers for data import

[lakshmi.anarayanan (Lakshmi Narayanan)]

I have paired-end demultiplexed Fastq data with filenames in the following format for a single sample.

ACA1-H_S3_L001_R1_001.fastq.gz

ACA1-H_S3_L001_R2_001.fastq.gz

I believe that I could use the Casava 1.8 paired-end demultiplexed fastq protocol for importing this data. However, in place of barcode sequence/ or a barcode identifier, what I have is S3 in this example.

I had 56 samples in my Illumina MiSeq run and in place of a barcode identifier what I have is S1 – S56 from sample #1- sample #56.
Are these S1- S56 series considered as barcode identifiers for their respective samples? I was expecting an i5 or i7 combination here. Please let me know.

Thank you,
Lakshmi

[thermokarst]

Hello @lakshmi.anarayanan!

@lakshmi.anarayanan

I believe that I could use the Casava 1.8 paired-end demultiplexed fastq protocol for importing this data.

Looks like it to me, too!

@lakshmi.anarayanan

Are these S1- S56 series considered as barcode identifiers for their respective samples?

Yep, look like it to me!

Keep us posted!

[lakshmi.anarayanan (Lakshmi Narayanan)]

Thank you, Matthew! The sequences could be imported successfully. So looks like I’m in the right track.