barcode.fastq.gz vs forward.fastq.gz

Hello. When using the command ‘demux emp-paired’, I received the error about sequence header lines containing ‘description fields’ but not barcode header lines.

The first few lines of my forward sequence file is:
@DGZN8DQ1:549:H7C23BCXX:2:1101:1087:1870 1:N:0:CGTCGTATGAAT

GANGGAGGATGCAAGTGTTATCCGGAATCACTGGGCGTAAAGCGTCTGTAGGTGGTTTGTTAAGTCAACTGTTAAATCTTGAGGCTCAACTTCAAATCAGCAGTCGAAACTATCAGACNNNNNNNNNNNNNNNNNNNNNNNNNNNTNNNG

+

DD#<<EHHIIIIEHIHIIIIIIIIIIIIIIIIIIIIGHIIIHHHGHIIIIHIGHIIIIIIIIIIIIIIIIHHHIIIIIIIIIIIIIIIIIIIIIIHIHEHHGHHIIIIIIIIHIIIHH################################

And for my barcode:

@DGZN8DQ1:549:H7C23BCXX:2:1101:1087:1870

CGTCGTATGAAT

+

############

If not mistaken, the main reason for this error is probably the 1st line of the barcode missing “1:N:0:CGTCGTATGAAT”. If this is correct, how do I make the barcode headers have corresponding description fields to the sequence headers? Cheers!

A post was merged into an existing topic: Errors: Demultiplexing with Mapping File