Bad reverse plot quality

Hello everyone,

I've imported all of my data and have reached the quality scores plot. It appears that my reverse sequences are showing poor quality, but I'm unsure why this has occurred.

Does anyone have any insights into why this might be happening, and how I could go about solving this issue?

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Hello Jara,

Thank you for posting that graph. That's extremely helpful.

For a run that looks like that, I would expect some of Illumina's run quality checks to also fail.
Like, showing unbalanced clustering densities or bubbles introduced during the 2nd half of the run.

Do you have run quality data from the Instrument to confirm?


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