I'm using Qiime 2021.11 version and want to assign taxonomy to some individual sequences files. I performed 16S v3/v4 sequencing through the company GENEWIZ for some bacterial isolates, and besides providing me with the raw forward and reverse reads, they also provided me with 1 consensus sequence filed (already merged and trimmed) for each sample. So starting with this sample I did the following:
qiime tools import --input-path 132_1B.fasta --output-path venom.qza --type 'FeatureData[Sequence]'
qiime feature-classifier classify-consensus-blast --i-query venom.qza --i-reference-reads silva-138-99-seqs.qza --i-reference-taxonomy silva-138-99-tax.qza --o-classification blast-venom.qza
qiime metadata tabulate --m-input-file venom_dada2_taxonomy.qza --o-visualization venom_dada2_taxonomy.qzv
However, I believe that by assigning taxonomy this way I am doing it to 99% similarity, is that correct? If so, how could I do to do to 98 or 97% similarity?
I can get down to genera for most samples but not species and I'm wondering if I could get closer by loosening my similarity restrictions.