I'm just wondering if full-length weighted taxonomy references made using clawback (as found in the readytowear collections ex. readytowear/data/gg_13_8/full_length at master · BenKaehler/readytowear · GitHub ) are ok to use with targeted regions like V3-V4. I know with non-weighted classifiers they …

Good questions, @Mehrbod_Estaki ! [image] Mehrbod_Estaki: I know with non-weighted classifiers they performs fine enough, perhaps a little bit worse than if they were trained on the specific region, but I’m wondering if this has been tested with bespoke classifiers? Safe to use? Yes. I am sti…

Just a minor addendum to @Nicholas_Bokulich 's excellent response. Currently, if you wish to use the full length weights, you have to train your classifier using the full length reference sequences and taxonomy. We are considering changing this in a future release (to make it possible to train a cl…

Thank you both for the thorough responses and this awesome plugin!

QIIME 2 Forum

Are full length 16S bespoke taxonomy references ok to use on specific regions?

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