ANCOM-volcano plot

annsantos · January 28, 2020, 11:40am

Hi,
I'm having problems with the interpretation of the volcano plot. Is this plot normal? Most of them are near the w=0.

If anyone could help me i would be grateful.

Ana Santos

jwdebelius · January 28, 2020, 12:51pm

Hi @annsantos,

It looks like your W value is 1, which says that the ASV changes with regard to one organism (which looks like it’s your one point at 100 and a second at 200).

Remember that W is calculated here as the number of tests where the ratio between two features is significant.

Best,
Justine

annsantos · January 28, 2020, 1:13pm

Thank you for your reply.

My W isn’t 200? The only that was statistically significant?

Ana Santos

jwdebelius · January 28, 2020, 1:24pm

Hi @annsantos,

If I look at the image you posted (d, you have something that’s a class Synergista with the W given (W=200). That would be the only feature where you rejected the null hypothesis enough times, making it the ANCOM equivalent of “significant”.

Best,
Justine

system · February 28, 2020, 7:24pm

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