ANCOM-BC2 action not available in QIIME2 composition plugin

Technical Support
1

Hello everyone,

I am trying to run ANCOM-BC2 in QIIME2 using the following command:



qiime composition ancombc2 \
  --i-table feature-table.qza \
  --m-metadata-file metadata.tsv \
  --p-fixed-effects-formula "metadata_column" \
  --o-ancombc2-output ancombc2-results.qza

However, I receive the following error:



Error: QIIME 2 plugin 'composition' has no action 'ancombc2'.
Did you mean 'ancombc'?

When I replace ancombc2 with ancombc, I receive different errors, presumably because the two actions use different parameters.

I am currently using the qiime2-amplicon-2024.10 environment.

Could anyone please advise whether ANCOM-BC2 is available in this QIIME2 version, or whether I need to install or update to a different version or environment?

Thank you very much for your help.

2

HI @R44H88,

The ancombc2 functionality was not added until the rachis-qiime2-2025.4 release. See the associated release announcement here.

I'd suggest downloading the latest rachis-qiime2-2026.7 release, as there have been two years of updates and improvements since qiime2-amplicon-2024.10.

-Cheers!

3

Hi @SoilRotifer Thank you so much! That was very helpful. I'll update my QIIME2 environment