Hello again @gmdouglas
Thanks so much. It worked. For benefit of others, who may be stuck here, this is what I did…
qiime tools export
–input-path $FILTER/rep-seqs-dada2.qza
–output-path $FILTER/export
Then
module avail picrust2
Then
module load picrust2/2.3.0
Then
picrust2_pipeline.py
-s $FILTER/export/rep-seqs-dada2.fasta
-i $FILTER/exported-feature-table_biom/feature-table.biom
-o $PIC2/picrust2_out_pipeline
-p 1
Then
add_descriptions.py -i $PIC2/picrust2_out_pipeline/EC_metagenome_out/pred_metagenome_unstrat.tsv.gz -m EC
-o $PIC2/picrust2_out_pipeline/EC_metagenome_out/pred_metagenome_unstrat_descrip.tsv.gz
add_descriptions.py -i $PIC2/picrust2_out_pipeline/KO_metagenome_out/pred_metagenome_unstrat.tsv.gz -m KO
-o $PIC2/picrust2_out_pipeline/KO_metagenome_out/pred_metagenome_unstrat_descrip.tsv.gz
add_descriptions.py -i $PIC2/picrust2_out_pipeline/pathways_out/path_abun_unstrat.tsv.gz -m METACYC
-o $PIC2/picrust2_out_pipeline/pathways_out/path_abun_unstrat_descrip.tsv.gz
Thanks very much once again,
Best wishes