I have Nanopore generated 16S fasta file which is demultiplexed and basecalled. For the taxonomic identification of such reads, can I use QIIME2. If yes, how should I begin because I am new to the program. I have used LAST alignment tool for such reads but want to cross check my data with the QIIME2.
Welcome back to the forums!
Try using this: GitHub - MaestSi/MetONTIIME: A Meta-barcoding pipeline for analysing ONT data in QIIME2 framework
It's not officially supported, but it's a good place to start with ONT data.
This topic was automatically closed 31 days after the last reply. New replies are no longer allowed.