I'm relatively new to the field of microbiome research and could use some guidance regarding a general question I have. I have a set of 15 samples consisting of pure bacteria sequences along a quality average score and length of contig for each sample sequence, and I'm interested in determining their respective phyla. I was wondering if someone could provide insight into the appropriate analysis and methods to accomplish this. Additionally, I would like to know if Qiime is a suitable tool for this task.
Any help or suggestions would be greatly appreciated!
I was chatting with Chloe about this. I'm a little confused about your goals here. Can you tell us more?
This sounds cool!
Because you are working with bacterial isolates, it should be easy to do genomic sequencing and then assemble these into genomes with either shotgun or long-read sequencing. Or both!
Soxs genes
How did you pick out this gene? Is there a paper you can share with us?