An error was encountered while running DADA2 in R

[thermokarst]

Hi @ashutosh!

@ashutosh

Can you suggest what wrong with my files or command?

Yep, the error above:

@ashutosh

Error in isBimeraDenovoTable(unqs[[i]], ..., verbose = verbose) : Input must be a valid sequence table. Calls: removeBimeraDenovo -> isBimeraDenovoTable In addition: Warning message: In is.na(colnames(unqs[[i]])) : is.na() applied to non-(list or vector) of type 'NULL'

indicates that your forward and reverse reads are not joining. Options include adjusting the trim/trunc params, or proceeding with just the forward or reverse reads on their own.