An error was encountered while running DADA2 in R (return code 1)

[Nicholas_Bokulich]

Hi @wang_leon,
See here for a description of this error message and how to troubleshoot:

Plugin error from dada2: An error was encountered while running DADA2 in R (return code 1), please inspect stdout and stderr to learn more

Hi there @steffi! This error comes from DADA2 when none of your paired-end reads were successfully joined. Perhaps your trimming parameters were too aggressive? How big is the region your primers are targeting?

Good luck! Let us know if you are able to fix by changing truncation parameters (note those values do not need to be the same)