Hi @kkcool! It looks like your forward and reverse reads might be identical - please see this thread, which was a pretty similar situation. Can you provide some more details about your source data:
- How did you import it? Copy-and-paste your import command, please.
- How is the data formatted before import? Is it demultiplexed? Can you screenshot the folder with your sequences please.
- Have the primers been removed, etc?
Thanks! 