alpha rarefaction curves not appearing

I am trying to plot my alpha rarefaction curves at a depth of 10,000 to select a rarefaction depth for my database. The table was generated using dada2 and has been filtered to remove mitochondria, chloroplasts, and unassigned reads.
I have never had an issue with getting curves to appear, but for some reason every time I make the plot it is blank, only showing samples distributed along the y-axis. The ASV table looks normal and has over 3 million reads. Any recommendations?
Here is the code I ran:

qiime diversity alpha-rarefaction
--i-table table.qza
--i-phylogeny rooted-tree.qza
--p-max-depth 10000
--m-metadata-file metadata.txt
--o-visualization alpha-rarefaction-r10000.qzv

Hi @Wes_Neely,
This was a bug with our most recent release, but its fixed now!
please uninstall and re-install the newest release of qiime2 and this should fix the issue!

Hey Chloe, I am running 2024.5 which is the newest release (as far as I can tell)!

Hey @Wes_Neely,

You'll want to see 2024.5.1 in the list for diversity when you run

qiime info

Otherwise, you are missing the patch that fixes this.

2 Likes