I am having trouble generating the alpha diversity. So far everything else has worked, but when I do the command:
qiime diversity alpha-group-significance
I receive this message:
Plugin error from diversity:
Metadata does not contain any columns that satisfy this visualizer's requirements. There must be at least one metadata column that contains categorical data, isn't empty, doesn't consist of unique values, and doesn't consist of exactly one value.
Debug info has been saved to /tmp/qiime2-q2cli-err-2lea_oet.log
I do not understand why it cannot read the metadata, since it did in the previous step just fine.
Attached is the .tsv file that I uploaded.
The command below was also run as suggested in another post to generate a table of alpha diversity values found in the Faith's PD vector:
qiime metadata tabulate
And this is the output:
This is consistent with the sample depth that was set to exclude two of the samples that had sequence counts of 0, so we know that we still have the 14 samples that we intended to have.