Alpha Group Significance Error Message

Currently running an alpha group significance with the code
qiime diversity alpha-group-significance
--i-alpha-diversity ./core-metrics-results/shannon_vector.qza
--m-metadata-file ./Testmetadata.tsv
--o-visualization ./core-metrics-results/shannon_vector.qzv

Keep getting the error message saying our metadata file does not have the column does satisfy the visualizer's requirement

This is our metadata file

This is the error message

Plugin error from diversity:

Metadata does not contain any columns that satisfy this visualizer's requirements. There must be at least one metadata column that contains categorical data, isn't empty, doesn't consist of unique values, and doesn't consist of exactly one value.

Debug info has been saved to /tmp/qiime2-q2cli-err-50shtlg_.log

Hi @ido1,

Welcome to the :qiime2: forum!

Do you mind sharing your shannon_vector.qza and Testmetadata.tsv files for me to examine? DM is fine if you'd prefer not to share them publicly. Thanks! :lizard:

shannon_vector.qza (52.5 KB)
Testmetadata.tsv (299 Bytes)

Hey @ido1 it seems as if your sampling depth is too deep, it only retains 2 samples; 98039 & W190020. I would suggest looking at your interactive feature table to determine a more appropriate sampling depth to retain more samples.

Check out this youtube video to help determine a sampling depth.

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