Currently running an alpha group significance with the code
qiime diversity alpha-group-significance
--i-alpha-diversity ./core-metrics-results/shannon_vector.qza
--m-metadata-file ./Testmetadata.tsv
--o-visualization ./core-metrics-results/shannon_vector.qzv
Keep getting the error message saying our metadata file does not have the column does satisfy the visualizer's requirement
This is our metadata file
This is the error message
Plugin error from diversity:
Metadata does not contain any columns that satisfy this visualizer's requirements. There must be at least one metadata column that contains categorical data, isn't empty, doesn't consist of unique values, and doesn't consist of exactly one value.
Debug info has been saved to /tmp/qiime2-q2cli-err-50shtlg_.log
Hi @ido1,
Welcome to the 
forum!
Do you mind sharing your shannon_vector.qza and Testmetadata.tsv files for me to examine? DM is fine if you'd prefer not to share them publicly. Thanks! 
Hey @ido1 it seems as if your sampling depth is too deep, it only retains 2 samples; 98039 & W190020. I would suggest looking at your interactive feature table to determine a more appropriate sampling depth to retain more samples.
Check out this youtube video to help determine a sampling depth.
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