alpha diversity with manifest

Hi, i'm in troubles with aplha diversity.
I imported my seqs with manifest in Phred33V2 so i dont have a metadata file
but when i run the command:

qiime diversity alpha-group-significance
--i-alpha-diversity metricas/faith_pd_vector.qza
--m-metadata-file manifest.tsv
--o-visualization metricas/faith-pd-group-significance.qzv
i have the following error:

" metadata does not contain any columns that satisfy this visualizer requirement..."

my manifest file is:

then, what should i do?
i have to make another file? or i have to add columns to my manifest?

In order to run alpha diversity group significance plugin, your metadata file should contain information about samples, for example, treatment, location, health and so on. Each group should be represented multiple times and there should be more than 1 group.
You can add descriptions to your manifest file. Also, it would be better to rerun core-metrics plugin with a new metadata file since it will allow you to group your sample in emperor visualization for beta diversity.


Thank you so much. I added the column of location to manifest and i didn't get any error. That was very helpful :+1::sunglasses::sunglasses:

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