Alpha Diversity Analysis Metadata assistance

[Megan_Whisonant]

Qiime 2 Version: 2022.2 installed via conda

Initial command Line:
qiime diversity alpha-group-significance
--i-alpha-diversity diversity-metrics-results/faith_pd_vector.qza
--m-metadata-file sample-metadata.tsv
--o-visualization diversity-metrics-results/faith-pd-group-significance.qzv

Error message:
Metadata does not contain any columns that satisfy this visualizer's requirements. There must be at least one metadata column that contains categorical data, isn't empty, doesn't consist of unique values, and doesn't consist of exactly one value.

So, in another qiime2 forum dealing with the same error message, I tried the following:

Command Line:
qiime metadata tabulate
--m-input-file faith_pd_vector.qza
--o-visualization core-metrics-results/faith_pd_vector.qzv

From there, I used the visualization file and this was generated (two columns on the left):

Then, my error message was:
The Following IDs are not present in the metadata: 'C1', 'C2', 'C3', 'C4', 'C5', 'E1', 'E2', 'E3', 'E4', 'E5'

Can someone please point me in the right direction to generate the faith-pd-group-significance.qzv?

Thanks!

[timanix (Timur Yergaliyev)]

Hello!
As far I can see two issues with your data.

Issue 1.

@Megan_Whisonant

The Following IDs are not present in the metadata: 'C1', 'C2', 'C3', 'C4', 'C5', 'E1', 'E2', 'E3', 'E4', 'E5'

Looks like you have sample IDs in faith_pd_vector.qza that are missing in the metadata. Double check that you are using the same metadata file from the project (sample IDs should be the same).

Issue 2.
Your metadata also should have a column with catergorical data, such as treatment, health, sampling site, age, etc. It is necessary for stat analysis, since you need to column that can group your samples to perform comparissons.