Hello,

Concerning boxplots for alfa diversity and the command:

qiime diversity alpha-group-significance.

I am looking at my nice boxplots as I write this.

I am wondering if there is any way to generate boxplots which has two categorial variables (for example: healthy/infected on x-axis and sampletype1-sampletype2 indicated as boxes next to each other in diffrent color - This means 4 boxes in total) in qiime2.

If not, I am wondering how I can obtain the alfa indices from each sample (which is the foundation for the qiime2 boxplots that I am looking at) to take this further to for example R.

I have paired-end illumina Miseq-data of 162 (v3-v4) and use version 2021.8.

The question I am trying to look into is, if I can gain more/different information about disease x by sampletype 1 or 2.

Thank you very much.