Hi I want to eliminate 15 samples from the table and run the analysis.
I tried running qiime like below:
qiime feature-table filter-samples
–m-metadata-file HCmetadata-new.tsv \
–p-where ““sample-id” NOT IN (‘sample21’, ‘sample25’, ‘sample26’, ‘sample27’, ‘sample29’, ‘sample39’, ‘sample55’, ‘sample57’, ‘sample59’, ‘sample60’, ‘sample76’, ‘sample77’, ‘sample85’, ‘sample87’, 'sample112)” \
and I keep running into this error no matter what I change and try.
"There was an issue with loading the file as metadata:
Metadata file path doesn’t exist, or the path points to something other than a file. Please check that the path exists, has read permissions, and points to a regular file (not a directory):
There may be more errors present in the metadata file. To get a full report, sample/feature metadata files can be validated with Keemei: https://keemei.qiime2.org
But it’s the same metadata that I’ve used multiple times in analyses like alpha and beta diversity. Why is it not working in this filter-sample command?
I’m attaching my metadata as well.