Running external command line application(s). This may print messages to stdout and/or stderr.
The command(s) being run are below. These commands cannot be manually re-run as they will depend on temporary files that no longer exist.

Command: run_aldex2.R /var/folders/9p/rn5lzh1n28ldm2rqlxqjp8xc0000gn/T/tmppxm48n0w/input.tsv.biom /var/folders/9p/rn5lzh1n28ldm2rqlxqjp8xc0000gn/T/tmppxm48n0w/input.map.txt Identifier 128 t all /var/folders/9p/rn5lzh1n28ldm2rqlxqjp8xc0000gn/T/tmppxm48n0w/output.summary.txt

R version 3.5.1 (2018-07-02) 
[1] "aldex.clr: generating Monte-Carlo instances and clr values"
[1] "operating in serial mode"
[1] "computing center with all features"
[1] "aldex.ttest: doing t-test"
[1] "running tests for each MC instance:"
|--Error in apply(data[, grp1], 1, sd) : dim(X) must have a positive length
Calls: aldex -> aldex.ttest -> t.fast -> apply
Execution halted
Traceback (most recent call last):
  File "/opt/miniconda2/envs/qiime2-2020.6/lib/python3.6/site-packages/q2_aldex2/_method.py", line 59, in aldex2
    run_commands([cmd])
  File "/opt/miniconda2/envs/qiime2-2020.6/lib/python3.6/site-packages/q2_aldex2/_method.py", line 21, in run_commands
    subprocess.run(cmd, check=True)
  File "/opt/miniconda2/envs/qiime2-2020.6/lib/python3.6/subprocess.py", line 438, in run
    output=stdout, stderr=stderr)
subprocess.CalledProcessError: Command '['run_aldex2.R', '/var/folders/9p/rn5lzh1n28ldm2rqlxqjp8xc0000gn/T/tmppxm48n0w/input.tsv.biom', '/var/folders/9p/rn5lzh1n28ldm2rqlxqjp8xc0000gn/T/tmppxm48n0w/input.map.txt', 'Identifier', '128', 't', 'all', '/var/folders/9p/rn5lzh1n28ldm2rqlxqjp8xc0000gn/T/tmppxm48n0w/output.summary.txt']' returned non-zero exit status 1.

During handling of the above exception, another exception occurred:

Traceback (most recent call last):
  File "/opt/miniconda2/envs/qiime2-2020.6/lib/python3.6/site-packages/q2cli/commands.py", line 329, in __call__
    results = action(**arguments)
  File "<decorator-gen-523>", line 2, in aldex2
  File "/opt/miniconda2/envs/qiime2-2020.6/lib/python3.6/site-packages/qiime2/sdk/action.py", line 245, in bound_callable
    output_types, provenance)
  File "/opt/miniconda2/envs/qiime2-2020.6/lib/python3.6/site-packages/qiime2/sdk/action.py", line 390, in _callable_executor_
    output_views = self._callable(**view_args)
  File "/opt/miniconda2/envs/qiime2-2020.6/lib/python3.6/site-packages/q2_aldex2/_method.py", line 63, in aldex2
    " and stderr to learn more." % e.returncode)
Exception: An error was encountered while running ALDEx2 in R (return code 1), please inspect stdout and stderr to learn more.