Running external command line application(s). This may print messages to stdout and/or stderr. The command(s) being run are below. These commands cannot be manually re-run as they will depend on temporary files that no longer exist. Command: run_dada_single.R /tmp/qiime2-archive-ff9pnfyr/20bf52fd-0be1-4cfc-82c4-1411f8c49dc2/data /tmp/tmpvlhq3iey/output.tsv.biom /tmp/tmpvlhq3iey/track.tsv /tmp/tmpvlhq3iey 120 0 2.0 2 Inf consensus 1.0 1 1000000 NULL 16 R version 3.5.1 (2018-07-02) Loading required package: Rcpp Traceback (most recent call last): File "/home/ubuntu/miniconda3/envs/qiime2-2019.4/lib/python3.6/site-packages/q2_dada2/_denoise.py", line 152, in _denoise_single run_commands([cmd]) File "/home/ubuntu/miniconda3/envs/qiime2-2019.4/lib/python3.6/site-packages/q2_dada2/_denoise.py", line 36, in run_commands subprocess.run(cmd, check=True) File "/home/ubuntu/miniconda3/envs/qiime2-2019.4/lib/python3.6/subprocess.py", line 418, in run output=stdout, stderr=stderr) subprocess.CalledProcessError: Command '['run_dada_single.R', '/tmp/qiime2-archive-ff9pnfyr/20bf52fd-0be1-4cfc-82c4-1411f8c49dc2/data', '/tmp/tmpvlhq3iey/output.tsv.biom', '/tmp/tmpvlhq3iey/track.tsv', '/tmp/tmpvlhq3iey', '120', '0', '2.0', '2', 'Inf', 'consensus', '1.0', '1', '1000000', 'NULL', '16']' died with . During handling of the above exception, another exception occurred: Traceback (most recent call last): File "/home/ubuntu/miniconda3/envs/qiime2-2019.4/lib/python3.6/site-packages/q2cli/commands.py", line 311, in __call__ results = action(**arguments) File "", line 2, in denoise_single File "/home/ubuntu/miniconda3/envs/qiime2-2019.4/lib/python3.6/site-packages/qiime2/sdk/action.py", line 231, in bound_callable output_types, provenance) File "/home/ubuntu/miniconda3/envs/qiime2-2019.4/lib/python3.6/site-packages/qiime2/sdk/action.py", line 365, in _callable_executor_ output_views = self._callable(**view_args) File "/home/ubuntu/miniconda3/envs/qiime2-2019.4/lib/python3.6/site-packages/q2_dada2/_denoise.py", line 187, in denoise_single band_size='16') File "/home/ubuntu/miniconda3/envs/qiime2-2019.4/lib/python3.6/site-packages/q2_dada2/_denoise.py", line 163, in _denoise_single " and stderr to learn more." % e.returncode) Exception: An error was encountered while running DADA2 in R (return code -9), please inspect stdout and stderr to learn more.